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ISOLDA BUDNIK O MARCELA FERRÉS G TRINIDAD PARDO T JAVIERA EDWARDS T GONZALO LABARCA T FELIPE REYES Z CONSTANZA MARTINEZ-VALDEBENITO LUISA MONTECINOS P CECILIA PERRET P

Abstract

Objective: To assess the performance of multiplex-PCR for diagnosis of respiratory viruses in parallel with direct fluorescence assay (DFA). We assessed the performance and co-infection diagnosis of molecular respiratory panel PCR (MRP-PCR) and DFA in hospitalized and outpatients. Results: 8535 samples were included, 1792 tested by MRP-PCR (46.9% positive) and 6743 by DFA (35.1% positive). MRP-PCR diagnosed co-infection in 21.3% and DFA in 1.8% of the samples. Rhinovirus was the most common virus in any age group. In 210 patients both tests were done; 100 were positive by MRP-PCR and 18 by DFA. Positive concordance value was 6.2%. 85 samples were positive only by MRP-PCR and in 42 of them only novel respiratory viruses were identified. Performance of MRP-PCR was statistically significant compared DFA for traditional respiratory viruses. Discussion: Multiplex PCR has shown better sensitivity, may expand the etiologic spectrum of respiratory infections and detect a higher number of co-infections.

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BUDNIK O, I., FERRÉS G, M., PARDO T, T., EDWARDS T, J., LABARCA T, G., REYES Z, F., MARTINEZ-VALDEBENITO, C., MONTECINOS P, L., & PERRET P, C. (2016). Contribution of multiplex respiratory molecular panel to the diagnosis of acute respiratory infections. Revista Chilena De Enfermedades Respiratorias, 32(4), 224–232. Retrieved from https://revchilenfermrespir.cl/index.php/RChER/article/view/256